LOCUS       sequence-51772-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TAX1BP3
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             515..532
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(585..603)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      599..646
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(770..789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat ggtgcaaaga gttgaaattc
      121 acaagctgcg tcaaggtgag aacttaatcc tgggtttcag cattggaggt ggaatcgacc
      181 aggacccttc ccagaatccc ttctctgaag acaagacgga caagggtatt tatgtcacac
      241 gggtgtctga aggaggccct gctgaaatcg ctgggctgca gattggagac aagatcatgc
      301 aggtgaacgg ctgggacatg accatggtca cacacgacca ggcccgcaag cggctcacca
      361 agcgctcgga ggaggtggtg cgtctgctgg tgacgcggca gtcgctgcag aaggccgtgc
      421 agcagtccat gctgtccgaa accgaagttt gacagtaaag gtggatacgg atccgaattc
      481 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      541 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      601 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      661 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      721 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      781 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      841 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      901 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
//
