LOCUS       sequence-51775-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PGAM5
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(710..730)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(755..772)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctttgtag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttgtcgacgg agctcgaatt cggatccgta tccaccttta ctgtcaggat cgagtgatct
      121 tgtcgggagg catgaacccc gtgtccccga gggtcctgag cgcaactcgg ccgttgggtc
      181 ggatcaccag gtgggtgatg ctgccattat tgagggagag ccggagccag ccttcaggag
      241 gaaactgcag tgctctgcac acgatgtagc ggatgacgtt ggcgtgacag atgaagatct
      301 cgtaactgtc ctcctcctgc ctggcatctg cgcggtggat gtagttccgg aaggcggcct
      361 cgatccgggc tccgtcttcg taatactgca cagcttccgg cttccaatga gacacgggcg
      421 ggtctggctc gatgggggcg ccttcccgca gcagatctgt gctgactttg cagacgcctg
      481 gcaggtgccg gctgatgata tcggtggtct ctatggcgcg cgtcatagac gaatggacga
      541 ttttattaaa cttcaacccc aagcttgcca ggcggagccc agtgagttca gcctgctccc
      601 gacccagcgg ggtcagagtg cggtccttct ccagggagcc atccacgtgg tactgggaat
      661 gcctgatgag gaagatgtgc cgcgtggcct tggctttgta gtggtccatg gattggaagt
      721 acaggttctc ggtacccaga tctacaccag aagaatgatg atgatgatgg tgcatatgta
      781 tatctccttc ttaaagttaa caatattt
//
