LOCUS       sequence-51796-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pDDGFP-A
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(28..36,37..51)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(200..218)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     468..487
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     662..685
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc
       61 tcgagaatgg cgagcaagag cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
      121 ttggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgccct
      181 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
      241 tgggacatcc tgtcccccct gatcatgtac ggctccaaga tgtacgtgaa gcaccccgcc
      301 gacgtccccg attacatgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      361 cacttcgagg acggcggtct ggtgaccgcg acacaggact cctccctgca ggacggcacg
      421 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      481 aagaagacct tgggctggga ttatgccacc gagcgcctgt accccgaaga aggcgtgctg
      541 aagggcgagc tcctggggcg cctgaagctg aaggacggcg gcctcaacct ggtggagtcc
      601 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactactt cgtggacacc
      661 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
      721 gagggccgcc accacctggg gcatggtaca ggtagcacag gcagcggtag ctcc
//
