LOCUS       sequence-51797-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pDDGFP-B
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             16..25
                     /estimated_length=10
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(94..102,103..117)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(272..290)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     540..559
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     734..757
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttgggctaac aggagnnnnn nnnnntgggg ggttctcatc atcatcatca tcatnnnnnn
       61 nctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat
      121 ccgagcggta ccgcctcctc cgaggacaac aacatggcca ccatcaaaga gttcatgcgc
      181 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
      241 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
      301 ctgccctttg cctgggacat cctgtccccc cagatcatgt acggctccaa agcgtacgtg
      361 aggcaccccg ccgacatccc cgattacaag aagctgccct tccccgaggg cttcaagtgg
      421 gagcgcgtga tgaacttcga agacggcggt ctggtgaccg ttacccagga ctcctccctg
      481 caggacggca cgctgatctg caaggtgaag attcgcggca ccaacttccc ccccgacggc
      541 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagatgct gtaccccaag
      601 gacggcgtgc tgaagggcca tagctatcag gccctgaagc tgaaggacgg cggccactac
      661 ctggtggagt tcgagaccat ctacatggcc aagaagcccg tgcaactgcc cggcgattac
      721 tgtgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
      781 tacgggcgct ccgagggccg ccaccgtctg ggcatggacg agcggtacaa gtaaaanctt
//
