LOCUS       sequence-51799-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pDDYFP-B
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(33..41,42..56)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     479..498
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac gataaggatc
       61 cgagcggtac cgcctcctcc gaggacaaca acatggccac catcaaagag ttcatgcgct
      121 tccaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
      181 agggccgccc ctacgagggc acccagaccg ccaaactgaa ggtgaccaag ggcggccccc
      241 tgcccttcgc ctgggacatc ctgtcccccc agatcatgta cggctccaaa gcgtacgtga
      301 ggcaccccgc cgacatcccc gattacaaga agctgccctt ccccgagggc ttcaggtggg
      361 agcgcgtgat gaacttcgaa gacggcggtc tggtgaccgt tacccaggac tcctccctgc
      421 aggacggcac gctggtctgc aaggtgaaga ttcgcggcac caacttcccc cccgacggcc
      481 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagatgctg taccccaaag
      541 acggcgtgct gaagggccat agctatcagg ccctgaagct gaaggacggc ggccactacc
      601 cggtggagtt cgagaccatc tacatggcca agaagcccgt gcaactgccc ggcgattact
      661 gtgtggtcac caagctggtc atcacctccc acaacgagga ctacaccatc gtggaacagt
      721 acgggcgctc cgagggccgc caccgtctgg gcatggacga gcggtacaag taaaagc
//
