LOCUS       sequence-51800-e        2526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pCalN-ddGFP-A
FEATURES             Location/Qualifiers
     source          1..2526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1952..1970)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2220..2239
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2414..2437
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggaaggga agtggttgct gtgtatgtta ctggtgcttg gaactgctat tgttgaggct
       61 catgatggac atgatgatga tgtgattgat attgaggatg accttgacga tgtcattgaa
      121 gaggtagaag actcaaaacc agataccact gctcctcctt catctcccaa ggttacttac
      181 aaagctccag ttccaacagg ggaagtatat tttgctgatt cttttgacag aggaactctg
      241 tcagggtgga ttttatccaa agccaagaaa gacgataccg atgatgaaat tgccaaatat
      301 gatggaaagt gggaggtaga ggaaatgaag gagtcaaagc ttccaggtga taaaggactt
      361 gtgttgatgt ctcgggccaa gcatcatgcc atctctgcta aactgaacaa gcccttcctg
      421 tttgacacca agcctctcat tgttcagtat gaggttaatt tccaaaatgg aatagaatgt
      481 ggtggtgcct atgtgaaact gctttctaaa acaccagaac tcaacctgga tcagttccat
      541 gacaagaccc cttatacgat tatgtttggt ccagataaat gtggagagga ctataaactg
      601 cacttcatct tccgacacaa aaaccccaaa acgggtatct atgaagaaaa acatgctaag
      661 aggccagatg cagatctgaa gacctatttt actgataaga aaacacatct ttacacacta
      721 atcttgaatc cagataatag ttttgaaata ctggttgacc aatctgtggt gaatagtgga
      781 aatctgctca atgacatgac tcctcctgta aatccttcac gtgaaattga ggacccagaa
      841 gaccggaagc ccgaggattg ggatgaaaga ccaaaaatcc cagatccaga agctgtcaag
      901 ccagatgact gggatgaaga tgcccctgct aagattccag atgaagaggc cacaaaaccc
      961 gaaggctggt tagatgatga gcctgagtac gtacctgatc cagacgcaga gaaacctgag
     1021 gattgggatg aagacatgga tggagaatgg gaggctcctc agattgccaa ccctagatgt
     1081 gagtcagctc ctggatgtgg tgtctggcag cgacctgtga ttgacaaccc caattataaa
     1141 ggcaaatgga agcctcctat gattgacaat cccagttacc agggaatctg gaaacccagg
     1201 aaaataccaa atccagattt ctttgaagat ctggaacctt tcagaatgac tccttttagt
     1261 gctattggtt tggagctgtg gtccatgacc tctgacattt tttttgacaa ctttatcatt
     1321 tgtgctgatc gaagaatagt tgatgattgg gccaatgatg gatggggcct gaagaaagct
     1381 gctgatgggg ctgctgagcc aggcgttgtg gggcagatga acgaggcagc tgaagagcgc
     1441 ccgtggctgt gggtagtcta tattctaact gtagcccttc ctgtgttcct ggttatcctc
     1501 ttctgctgtt ctggaaagaa acagaccagt ggtatggagt ataagaaaac tgatgcacct
     1561 caaccggatg tgaaggaaga ggaagaagag aaggaagagg aaaaggacaa gggagatgag
     1621 gaggaggaag gagaagagaa acttgaagag aaacagaaaa gtgatgctga agaagatggt
     1681 ggcactgtca gtcaagagga ggaagacaga aaacctaaag cagaggagga tgaaattttg
     1741 aacagatcac caagaaacag aaagccacga agagaggggg gtgcgtcagg cggcagcgga
     1801 tctggaccgg tcggtaccat ggtgagcaag agcgaggagg tcatcaaaga gttcatgcgc
     1861 ttcaaggtgc gcttggaggg ctccatgaac ggccacgagt tcgagatcga gggcganggc
     1921 gagggccgcc cttacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     1981 ctgcccttcg cctgggacat cctgtccccc ctgatcatgt acggctccaa gatgtacgtg
     2041 aagcaccccg ccgacgtccc cgattacatg aagctgtcct tccccgaggg cttcaagtgg
     2101 gagcgcgtga tgcacttcga ggacggcggt ctggtgaccg cgacacagga ctcctccctg
     2161 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     2221 cccgtaatgc agaagaagac cttgggctgg gattatgcca ccgagcgcct gtaccccgaa
     2281 gaaggcgtgc tgaagggcga gctcctgggg cgcctgaagc tgaaggacgg cggcctcaac
     2341 ctggtggagt ccaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     2401 ttcgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2461 tacgagcgct ccgagggccg ccaccacctg gggcatggta caggtagcac aggcagcggt
     2521 agctcc
//
