LOCUS       sequence-51802-e        1189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pTOM20-ddGFP-B
FEATURES             Location/Qualifiers
     source          1..1189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(627..645)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     895..914
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1089..1112
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gnnnnncncc ntggcctcga gaatggtggg tcggaacagc gccatcgccg ccggtgtatg
       61 cggggccctt ttcattgggt actgcatcta cttcgaccgc aaaagacgaa gtgaccccaa
      121 cttcaagaac aggcttcgag aacgaagaaa gaaacagaag cttgccaagg agagagctgg
      181 gctttccaag ttacctgacc ttaaagatgc tgaagctgtt cagaagttct tccttgaaga
      241 aatacagctt ggtgaagagt tactagctca aggtgaatat gagaagggcg tagaccatct
      301 gacaaatgca attgctgtgt gtggacagcc acagcagtta ctgcaggtct tacagcaaac
      361 tcttccacca ccagtgttcc agatgcttct gactaagctc ccaacaatta gtcagagaat
      421 tgtaagtgct cagagcttgg ctgaagatga tgtggaaggc ggtagcgggg atccaccggt
      481 cggtaccgcc tcctccgagg acaacaacat ggccaccatc aaagagttca tgcgcttcaa
      541 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      601 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
      661 ctttgcctgg gacatcctgt ccccccagat catgtacggc tccaaagcgt acgtgaggca
      721 ccccgccgac atccccgatt acaagaagct gcccttcccc gagggcttca agtgggagcg
      781 cgtgatgaac ttcgaagacg gcggtctggt gaccgttacc caggactcct ccctgcagga
      841 cggcacgctg atctgcaagg tgaagattcg cggcaccaac ttcccccccg acggccccgt
      901 aatgcagaag aagaccatgg gctgggaggc ctccaccgag atgctgtacc ccaaggacgg
      961 cgtgctgaag ggccatagct atcaggccct gaagctgaag gacggcggcc actacctggt
     1021 ggagttcgag accatctaca tggccaagaa gcccgtgcaa ctgcccggcg attactgtgt
     1081 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacgg
     1141 gcgctccgag ggccgccacc gtctgggcat ggacgagcgg tacaagtaa
//
