LOCUS       sequence-51815-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPSB1
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             801..818
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(871..889)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcagga gctccagggt
      121 ctggattact gcaagcccac ccggctggat ctgctactgg acatgccccc tgtgtcctat
      181 gatgtccagc tgctgcattc atggaacaac aacgaccgat cgctcaatgt ctttgtgaag
      241 gaggacgaca agctcatctt tcaccggcat ccggtggccc agagcacgga cgctatcagg
      301 ggcaaagtcg ggtatacccg tgggctgcac gtgtggcaga tcacgtgggc catgagacag
      361 cggggcacac acgccgtggt gggggtggcg acggcagacg cccccctgca ctctgtcggg
      421 tacacaaccc tcgtggggaa taaccacgag tcctggggct gggacttggg gcgcaaccgg
      481 ctctaccacg atggcaagaa ccagccaagc aaaacatacc cagcctttct ggaaccagat
      541 gagacattca ttgtccctga ctccttcctg gtagccctgg acatggacga cgggactctg
      601 agcttcattg tggatggaca gtacatggga gtggcttttc ggggactcaa gggcaaaaaa
      661 ctgtatcctg tagtgagtgc cgtctggggc cactgtgaga tccgaatgcg ctacttgaac
      721 ggactcgatc ccgagtgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
      781 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      841 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taacccc
//
