LOCUS       sequence-51817-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TWF2
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             574..591
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(644..662)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      658..705
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctgaaata ttttgtttac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgc ccctgcagcc
      121 tgaggcccag cgggcactcc agcagctcaa gcagaaaatg gtcaactaca tccagatgaa
      181 gctggaccta gagcgggaaa ccattgagct ggtgcacaca gagcccacgg atgtggccca
      241 gctgccctcc cgggtgcccc gagatgctgc ccgctaccac ttcttcctct acaagcacac
      301 ccatgagggc gacccccttg agtctgtagt gttcatctac tccatgccgg ggtacaagtg
      361 cagcatcaag gagcgaatgc tctactccag ctgcaagagc cgcctcctcg actccgtgga
      421 gcaggacttc catctggaga tcgccaagaa aattgagatt ggcgatgggg cagagctgac
      481 ggcagagttc ctcgacgacg aggtgcactg acagtaaagg tggatacgga tccgaattcg
      541 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      601 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      661 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      721 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct ccctttcgct
      841 ttcttccctt cctttctcgc cacgttcgcc g
//
