LOCUS       sequence-51819-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TH
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(92..115)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(116..133)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(134..154)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttgtcgacg gagctcgaat tcggatcctc acttatcgtc gtcatccttg taatcatggt
      121 ggtgatgatg gtgcgattgg aagtagaggt tctctgcgcc aatggcactc agcgcatggg
      181 caagggtgtc cagctcatcc tggacaccct ccagggagcg ccgcacggcc tgggggctgt
      241 ccagcacgtc gatggccagc gtgtacgggt cgaacttcac ggagaagggg cgctggatgc
      301 gtgaggcata gctcctgagc ttgtccttgg cgtcactgaa gctctcagac acgaagtaga
      361 ctgactggta cgtctggtct tggtagggct gcacggccgc agcctcaggg tcgaaggccc
      421 gaatctcagg ctcctcagac aggcagtgca ggagctcccc gtaggaggac agcagcccgg
      481 caccataggc cttcacctcc ccgttctgct tacacagccc gaactccacc gtgaaccagt
      541 acagcgtgga cagcttctca atttcctcat ccgaggcccc cagggacgcc aggccaatgt
      601 cctgcgagaa ctgcgcgaag gtgcggtcgg ccagcatggg cacgtgcccc agcagctcgt
      661 ggcagcagtc cggctcaggg gagtgcatgg gcgaggacgc gtggcggata tactgggtgc
      721 actggaacac gcggaaggcc aggctggcca ggaagtcccg ggcggacagc aggccggcca
      781 caggccgcag ctggaagccc gtgcgctcct tcaggaagcg ggagacgtcc tccagctggg
      841 ggatattgtc ttcccggtag ccgctgaagc gctccagcaa agcaaaggcc tccaggtgct
      901 ccccgcaggc gtgc
//
