LOCUS       sequence-51827-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MLYCD
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggacg
      121 agctgctgcg ccgcgcggtg ccgccgacgc cggcctacga gctgcgcgca gcaacaccgg
      181 cgcccgccga gggtcagtgc gcggacttcg tgagcttcta cggtgggctg gccgagacgg
      241 cccagcgggc cgaactgctg ggccgcctgg cgcggggctt cggcgtggac cacggccagg
      301 tggcggagca gagcgccggc gtgctccatc tgcgccagca gcagcgggag gcggcggtgc
      361 tgctgcaggc cgaggaccgg ctgcgctacg cgctggtgcc gcgctatcgc ggcctcttcc
      421 accacatcag caagctggac ggcggcgtgc gcttcctggt gcagctgcgg gccgacctgc
      481 tggaggcgca ggccctcaag ctggtggagg ggccggacgt ccgggaaatg aatggggtgc
      541 tgaaaggaat gctctcagaa tggttttcct ccgggttcct gaacctagaa cgggttacct
      601 ggcattcacc gtgtgaagtg cttcagaaaa tcagtgaggc tgaggctgtg catcctgtaa
      661 aaaactggat ggacatgaag cgccgcgttg ggccctacag aaggtgttac ttcttttctc
      721 actgttcgac ccctggggag cccctggtcg ttttgcacgt ggcactgact ggtgacatct
      781 ccagcaacat ccaggcaatc gtgaaggaac atcctccatc agaaacagca gctgctaaca
      841 aaatcactgc tgcgatcttt tattccatca gcttgaccca gcagggactc caaggggtgg
      901 agctgggaac attcct
//
