LOCUS       sequence-51828-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MCEE
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(495..515)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(540..557)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             568..590
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    605..629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(629..648)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(630..648)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(732..751)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga
       61 gctcgaattc ggatccgtat ccacctttac tgtcaagctt gctccagttc cacaaggact
      121 ccaccacagt ctttaggatg gagaaaaatc actggttttc catgtgctcc tattttgacc
      181 tcttcactta gactgcggat cttctttttt ttcaaatcca tcacagctgc attaatatta
      241 tccacctcga tgcagatgtg atgcattcct ccagccttgt ttttctgcag aaaacctgca
      301 attggactgt caagtcccaa tggatgaagc agttccatct tggtatttcc caggttgaca
      361 aaaacaacag atactccatg ttcaggaaga gggaccgctt cacttacctg ggcccccaga
      421 atattcttat aaaatgctgc agccttttcc aaatctggca ctgctatggc tacatggttg
      481 agtcgaccca gcatggattg gaagtacagg ttctcggtac ccagatctac accagaagaa
      541 tgatgatgat gatggtgcat atgtatatct ccttcttaaa gttaaacaaa attatttcta
      601 gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat
      661 cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
      721 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
      781 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
      841 gggcgccatc tccttgcat
//
