LOCUS       sequence-51829-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MCEE
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             560..577
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(630..648)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      644..691
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(815..834)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg
       61 tagatctggg taccgagaac ctgtacttcc aatccatgct gggtcgactc aaccatgtag
      121 ccatagcagt gccagatttg gaaaaggctg cagcatttta taagaatatt ctgggggccc
      181 aggtaagtga agcggtccct cttcctgaac atggagtatc tgttgttttt gtcaacctgg
      241 gaaataccaa gatggaactg cttcatccat tgggacttga cagtccaatt gcaggttttc
      301 tgcagaaaaa caaggctgga ggaatgcatc acatctgcat cgaggtggat aatattaatg
      361 cagctgtgat ggatttgaaa aaaaagaaga tccgcagtct aagtgaagag gtcaaaatag
      421 gagcacatgg aaaaccagtg atttttctcc atcctaaaga ctgtggtgga gtccttgtgg
      481 aactggagca agcttgacag taaaggtgga tacggatccg aattcgagct ccgtcgacaa
      541 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      601 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      661 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      721 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      781 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      841 ctcgccacgt
//
