LOCUS       sequence-51832-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACACA
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tctagaatat tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatcg gcttgcacct
      121 ggttaagcag ggtcgcgacc gtaagaaaat cgattctcaa cgcgacttta ctgtcgcgtc
      181 accagcagag ttcgtaacac gttttggcgg taataaggtg atcgagaaag ttttgattgc
      241 caacaatggc atcgctgcgg tcaagtgtat gcgcagtatt cgtcgctggt cgtacgaaat
      301 gttccgtaac gagcgcgcca tccgtttcgt ggtaatggtg acccctgagg acctcaaggc
      361 aaacgctgag tatattaaga tggccgacca ttacgttccg gtccccggcg gcccaaataa
      421 caataactat gcgaacgtcg agctgatctt ggatattgca aagcgcatcc ctgtgcaggc
      481 tgtttgggcc ggttggggcc acgcgagcga gaatccgaaa ctgcccgaac tcctcctcaa
      541 gaacggtatc gcttttatgg gcccaccttc ccaggccatg tgggcactgg gtgacaaaat
      601 tgcatcttca atcgtcgctc aaactgccgg cattccgaca ctgccctgga gtggtagcgg
      661 cttgcgtgtg gattggcagg aaaatgactt ctccaaacgc atcctgaacg taccccaaga
      721 gttgtacgaa aagggttatg ttaaagatgt cgacgatggc ctgcaggcag ccgaggaagt
      781 gggttatccc gtaatgatta aggcgtcgga gggcggcggc ggtaaaggca tccgtaaagt
      841 aaacaatgct gacgattttc cgaacctgtt
//
