LOCUS       sequence-51835-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TATDN1
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtaatcatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgct attacaggaa aaaatacttt aatagtattg
      181 ttatatagtg tattggctaa ttccagtgga tcctcatctc tcactgctga cattatctcc
      241 aatatttgaa ttatatggca gggttcattt ctgtctttta agcagtgccc actttcccac
      301 ttctttttgg taggaaatgc agttcttata tattttgatc cagcatgtgt acttttgact
      361 ccacaccaag gtgcatctgt ctcaatcatt aatttttcac taggaattga cttcaaaact
      421 tccaaattag cttcagtttt cagtgagcaa ccattaaatc ctatataaag atccaagtca
      481 atcaaagcag ctgctgcttc cttggtacca tcaaatgaat gcaccactcc ccctacacac
      541 cgatctctat ttcttttcgt tatgtccaaa aattcagcat gtgagtttcg acaatgaaga
      601 aacattggta attttgtttg ttctgacagt tcaaactgtt tttcaaaata tttgagttga
      661 gtatctttgg gacaaaactg cagtcggtca aaatcaagtc cgcattctcc tattgccaca
      721 actttccctt tattgttttc agcaagattt agcaactcct ttaagtaaag atcagggtta
      781 ttcttttcaa attcaccaca tcttgtagga tgacatccaa ctgtactgaa aaacatacca
      841 tttgtttgtg ccaaatgcag tgcatcttta ctgtcttgta gatttccacc tgtaatcata
      901 aactttttaa caccaatc
//
