LOCUS       sequence-51843-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 soybean APEX (IMS)
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..747
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 ggaaagtctt acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag
       61 aagctcagag gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc
      121 cactctgctg gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag
      181 caccctgccg aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg
      241 gagccactca aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc
      301 gttgttgccg ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac
      361 aagcctgagc caccaccaga gggtcgcttg cccgatgcca ctaagggttc tgaccatttg
      421 agagatgtgt ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg
      481 ggtcacacta ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct
      541 aatcctctta ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt
      601 ctccttcagc taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt
      661 gataaatatg cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag
      721 ctttccgagc ttgggtttgc tgatgcc
//
