LOCUS       sequence-51848-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PHKG2
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..249
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             256..276
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gagaattccc tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt ggataacaag ttcaacaagg agcgtcgaag agctcgccgt
      121 gaaattcgcc atctgccgaa cctgaaccgc gaacagcgtc gcgcatttat tcgcagcctg
      181 cgcgatgatc cgagccagag cgcgaacctg ctggcggaag cgaagaagct gaacgatgcg
      241 cagccgaagg gtaccgagaa cctgtacttc caatccatgg ggccggagga tgagctgccc
      301 gactgggccg ccgccaaaga gttttaccag aagtacgacc ctaaggacgt catcggcaga
      361 ggagtgagct ctgtggtccg ccgttgtgtt catcgagcta ctggccacga gtttgcggtg
      421 aagattatgg aagtgacagc tgagcggctg agtcctgagc agctggagga ggtgcgggaa
      481 gccacacggc gagagacaca catccttcgc caggtcgccg gccaccccca catcatcacc
      541 ctcatcgatt cctacgagtc ttctagcttc atgttcctgg tgtttgacct gatgcggaag
      601 ggagagctgt ttgactatct cacagagaag gtggccctct ctgaaaagga aaccaggtcc
      661 atcatgcggt ctctgctgga agcagtgagc tttctccatg ccaacaacat tgtgcatcga
      721 gatctgaagc ccgagaatat tctcctagat gacaatatgc agatccgact ttcagatttc
      781 gggttctcct gccacttgga acctggcgag aagcttcgag agttgtgtgg gaccccaggg
      841 tatctagcgc cagagatcct taaatgctcc atggatgaaa
//
