LOCUS       sequence-51849-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ANLN
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(544..564)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(589..606)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             617..639
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    654..678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(678..697)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(679..697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(781..800)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccttcgggct tgtagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc atttgtagca
      121 agcatcaggt tgccagaggc gaatatcaac aagaacttga ttgagttttt gcatccagag
      181 atcccgctct tctttagtat ctgcagacag ccagttcttg gtaacacaga gtgtgtccct
      241 gcattggctg acaagagtct ctcggtcatc ttctctttgt ggtcggacag taattaattc
      301 aaaagtgttg cgtcttgcac aaaattctct gttggctggt tctatctgac gactggtaca
      361 attagccaga tttatccttc ctatgggatt cttgcgtttc tcatcatctg gataagtcca
      421 ataagatata cagtttccag aaagaacaca ccatcttcga tgccaggcac caaaaccact
      481 aacatcttca aatatggtta gaaaacctct ttcttcaaca ctggaattca cttgacattt
      541 catggattgg aagtacaggt tctcggtacc cagatctaca ccagaagaat gatgatgatg
      601 atggtgcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg
      661 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga
      721 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      781 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
      841 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg ggggaactgt tgggcgccat
      901 ctcc
//
