LOCUS       sequence-51850-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ANLN
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             608..625
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(678..696)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      692..739
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(863..882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgaaatgtc
      121 aagtgaattc cagtgttgaa gaaagaggtt ttctaaccat atttgaagat gttagtggtt
      181 ttggtgcctg gcatcgaaga tggtgtgttc tttctggaaa ctgtatatct tattggactt
      241 atccagatga tgagaaacgc aagaatccca taggaaggat aaatctggct aattgtacca
      301 gtcgtcagat agaaccagcc aacagagaat tttgtgcaag acgcaacact tttgaattaa
      361 ttactgtccg accacaaaga gaagatgacc gagagactct tgtcagccaa tgcagggaca
      421 cactctgtgt taccaagaac tggctgtctg cagatactaa agaagagcgg gatctctgga
      481 tgcaaaaact caatcaagtt cttgttgata ttcgcctctg gcaacctgat gcttgctaca
      541 aatgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      601 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      661 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      721 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      781 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      841 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc ct
//
