LOCUS       sequence-51852-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CRYBB3
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..109
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             715..732
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(785..803)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      799..846
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctagaat aattttgttt aactttaaga aggagatata catatgcacc atcatcatca
       61 tcattcttct ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tggggggcag
      121 ctacaaggtg atcttgtacg aactagagaa cttccaaggc aaacgctgcg agctctcggc
      181 cgagtgcccc agcctgaccg acagcctgct ggagaaggtg ggctccatcc aagtggagtc
      241 cgggccgtgg ctggcatttg agtccagggc cttccgcggg gagcagtttg ttctggagaa
      301 gggggattat cctcgctggg atgcctggtc caacagccgt gatagtgaca gccttctgtc
      361 cctccggcct ctgaatattg atagtccaca tcacaagctg catctgtttg agaacccagc
      421 tttcagtggc cgcaagatgg agatagtgga tgatgacgtg cccagcctgt gggctcatgg
      481 cttccaggac cgtgtggcga gtgtccgtgc catcaacggg acgtgggttg gctatgagtt
      541 ccccggctac cgtgggcgcc agtacgtgtt tgagcggggc gagtaccgcc actggaatga
      601 gtgggacgcc agccagccgc agctgcagtc tgtgcgccgc atccgtgact gacagtaaag
      661 gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
      721 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      781 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      841 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      901 cata
//
