LOCUS       sequence-51854-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX3
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             480..497
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(550..568)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      564..611
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(735..754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tggctcccaa attagaggaa
      121 aggctcaaag tgctgacaaa taaagcttct gtgatgctct ttatgaaagg aaacaaacag
      181 gaagcaaaat gtggattcag caaacaaatt ctggaaatac taaatagtac tggtgttgaa
      241 tatgaaacat tcgatatatt ggaggatgaa gaagttcggc aaggattaaa agcttactca
      301 aattggccaa cataccctca gctgtatgtg aaaggggagc tggtgggagg attggatatt
      361 gtgaaggaac tgaaagaaaa tggtgaattg ctgcctatac tgagaggaga aaattgacag
      421 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      481 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      541 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      601 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      781 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      841 cctcgacccc aaaaaacttg attagg
//
