LOCUS       sequence-51856-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GYG1
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccctctgaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgacagatc
      121 aggcctttgt gacactaacc acaaacgatg cctacgccaa aggtgccctg gtcctgggat
      181 catctctgaa acagcacagg accaccagga ggctggtcgt gctcgccacc cctcaggtct
      241 cagactccat gagaaaagtt ttagagacag tctttgatga agtcatcatg gtagatgtct
      301 tggacagtgg cgattctgct catctaacct taatgaagag gccagagttg ggtgtcacgc
      361 tgacaaagct ccactgctgg tcgcttacac agtattcaaa atgtgtattc atggatgcag
      421 atactctggt cctagcaaat attgatgatc tttttgacag agaagaattg tcagcagcac
      481 cagacccagg gtggcctgac tgcttcaatt ccggagtctt cgtttatcag ccttcagttg
      541 aaacatacaa tcagctgttg catcttgctt ctgagcaagg tagttttgat ggtggggacc
      601 aaggcatact gaacacattt tttagcagct gggcaacaac agatatcaga aaacacctgc
      661 cgtttattta taacctaagc agcatctcta tatactccta cctcccggca tttaaagtgt
      721 ttggtgcaag tgccaaagtt gtgcatttcc tgggacgagt caaaccatgg aattatactt
      781 atgatcccaa aacaaaaagt gtcaaaagtg aggcccatga tcccaacatg actcatccag
      841 agtttctcat cctgtggtgg aacatcttta ccaccaacgt tttacctctg cttcaatgac
      901 agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aag
//
