LOCUS       sequence-51857-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HBP1
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(510..530)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(555..572)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             583..605
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    620..644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(644..663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(645..663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(747..766)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga
       61 gctcgaattc ggatccgtat ccacctttac tgtcaggcat cggggtgtcc aggaggtaga
      121 catacatcgc caatatgaac ttcacaacat ggaatgccat gctgtaccac agtcaagctt
      181 ggataaaatg atgaccaacc tttattttta acatagaaag ggtggtccag cttacactct
      241 acggtaagta aaccatcttc tactgtacca ggatcaaaag tcaacttcag tacagactcg
      301 ccaaatgata cactttcttc atgtgataac aactttagac catcagaacc atagcccttg
      361 tgaatgccca tttgaagatc ttcctcatta tcacagcctt cagctctagc aaaatcttca
      421 acatcttgcc attccttatt gcttccctta tgaaagcaca gtcgtgtgcc tttcaaaaaa
      481 cagtgccaga cagttgaagg ccaggacatg gattggaagt acaggttctc ggtacccaga
      541 tctacaccag aagaatgatg atgatgatgg tgcatatgta tatctccttc ttaaagttaa
      601 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta
      661 ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac
      721 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      781 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      841 ggccggggga ctgttgggcg ccatctcctt gcttgcacca ttccttgcgg cggcggtgct
      901 caacggcctc aacctactac tgggctgctt tcctaatgc
//
