LOCUS       sequence-51858-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HBP1
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             588..605
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(658..676)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      672..719
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(843..862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctagaata attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat gtcctggcct
      121 tcaactgtct ggcactgttt tttgaaaggc acacgactgt gctttcataa gggaagcaat
      181 aaggaatggc aagatgttga agattttgct agagctgaag gctgtgataa tgaggaagat
      241 cttcaaatgg gcattcacaa gggctatggt tctgatggtc taaagttgtt atcacatgaa
      301 gaaagtgtat catttggcga gtctgtactg aagttgactt ttgatcctgg tacagtagaa
      361 gatggtttac ttaccgtaga gtgtaagctg gaccaccctt tctatgttaa aaataaaggt
      421 tggtcatcat tttatccaag cttgactgtg gtacagcatg gcattccatg ttgtgaagtt
      481 catattggcg atgtatgtct acctcctgga caccccgatg cctgacagta aaggtggata
      541 cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc
      601 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      661 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      721 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
      781 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      841 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc ccccgtcaag
      901 ctctaaatcg ggggctcccc
//
