LOCUS       sequence-51859-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AMPH
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(723..743)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(768..785)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggcttggt tagcagccgg aatctcagtg gtggtggtgg tggtgctcga gtgcggccgc
       61 aagcttgtcg acggagctcg aattcggatc cgtatccacc tttactgtca gtcacccagt
      121 tttgtcatca cttcatacag tttgtggcaa agcaccgcaa tttccttatg aaacttggct
      181 tcaaggctgg agacgttttt gaaagtatta acataaaatc caactcgtct tgaccataat
      241 gatggtaact cttcttgtaa gtcaacgtta aactcttcaa acactttctg tgctttctga
      301 aattcttctt ctgccttaga gattcgactc tcatccttcc tcttggagct ctgcagagct
      361 tccagatggt ggcgggcact gtcatagtcc actagcttcc tgctgcgctt ggcgatgcga
      421 ttctttatgt caggaaattg ccccaggtag gtatccagtg ttagcaagga cccatccacg
      481 agtttttgat ggaagtcttc ccacagcaca tcacatttct caccaaccat tttcacatct
      541 tcccgcccat accagtcagg ctcatagact tcatgcagcg actctgtgag cttcatggag
      601 gcctcctgca tgcctttgat tgctgctaaa tatcctcgga gttctcgctg aagtctggta
      661 ccctctgctt cttgccgttt gaagttctgg acatattctt cgaactgttc gtcttttgtc
      721 atggattgga agtacaggtt ctcggtaccc agatctacac cagaagaatg atgatgatga
      781 tggtgcatat gtatatctcc tt
//
