LOCUS       sequence-51860-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AMPH
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             784..801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(855..873)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatga caaaagacga
      121 acagttcgaa gaatatgtcc agaacttcaa acggcaagaa gcagagggta ccagacttca
      181 gcgagaactc cgaggatatt tagcagcaat caaaggcatg caggaggcct ccatgaagct
      241 cacagagtcg ctgcatgaag tctatgagcc tgactggtat gggcgggaag atgtgaaaat
      301 ggttggtgag aaatgtgatg tgctgtggga agacttccat caaaaactcg tggatgggtc
      361 cttgctaaca ctggatacct acctggggca atttcctgac ataaagaatc gcatcgccaa
      421 gcgcagcagg aagctagtgg actatgacag tgcccgccac catctggaag ctctgcagag
      481 ctccaagagg aaggatgaga gtcgaatctc taaggcagaa gaagaatttc agaaagcaca
      541 gaaagtgttt gaagagttta acgttgactt acaagaagag ttaccatcat tatggtcaag
      601 acgagttgga ttttatgtta atactttcaa aaacgtctcc agccttgaag ccaagtttca
      661 taaggaaatt gcggtgcttt gccacaaact gtatgaagtg atgacaaaac tgggtgactg
      721 acagtaaagg tggatacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc
      781 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa aggaagctga
      841 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaacgggtc
      901 ttgaggggtt t
//
