LOCUS       sequence-51861-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ASPH
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(694..714)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(739..756)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             767..789
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    804..828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcaa attgctggaa ggctgcgtct
      121 ctgctgtggt gtcagttccg gatgccacac atccacgatg aatatcagcc ggaaagatga
      181 ggcatcctgc catacctcgt gctcaaagga gtcatcaaag atgagcacct tgccttcctc
      241 ccaggtcttg gtctcgttgg cacatcgaat cttgcagcct tccttgggaa tcaccaagcc
      301 caggtgcatt cggagcctgc agtttgtggg ccctgtgtgc ggccacacgt gagtcccggg
      361 gtgcatgatg gaatatttga tctgtcctct tctgcatcct gttgtctcgg ggaacttttc
      421 tagtaaggta caggttttag gagctccttt gcaggcattt tcatttcttc ttccttgctg
      481 ccacagcgtg aactggctcc agtccccttt ttccctcagg ttttcatcct caggcaggaa
      541 gagacctttg gctttatcca tcactgcaag gccttcatct cggattaact tccagtttct
      601 ttctaaagac tttactaact ctgtgtagcc cgtttctttt ggggtccacc aaggctgtgc
      661 tttcagtcca ttcacattgt agagtgagcg catggattgg aagtacaggt tctcggtacc
      721 cagatctaca ccagaagaat gatgatgatg atggtgcata tgtatatctc cttcttaaag
      781 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      841 cgtatttaat ttcgcg
//
