LOCUS       sequence-51862-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ASPH
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             758..775
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(828..846)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gcgctcactc tacaatgtga
      121 atggactgaa agcacagcct tggtggaccc caaaagaaac gggctacaca gagttagtaa
      181 agtctttaga aagaaactgg aagttaatcc gagatgaagg ccttgcagtg atggataaag
      241 ccaaaggtct cttcctgcct gaggatgaaa acctgaggga aaaaggggac tggagccagt
      301 tcacgctgtg gcagcaagga agaagaaatg aaaatgcctg caaaggagct cctaaaacct
      361 gtaccttact agaaaagttc cccgagacaa caggatgcag aagaggacag atcaaatatt
      421 ccatcatgca ccccgggact cacgtgtggc cgcacacagg gcccacaaac tgcaggctcc
      481 gaatgcacct gggcttggtg attcccaagg aaggctgcaa gattcgatgt gccaacgaga
      541 ccaagacctg ggaggaaggc aaggtgctca tctttgatga ctcctttgag cacgaggtat
      601 ggcaggatgc ctcatctttc cggctgatat tcatcgtgga tgtgtggcat ccggaactga
      661 caccacagca gagacgcagc cttccagcaa tttgacagta aaggtggata cggatccgaa
      721 ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      781 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      841 actagcataa cccccttggg gcctcta
//
