LOCUS       sequence-51863-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD1
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(506..526)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(551..568)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             579..601
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    616..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(640..659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(641..659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(743..762)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttattg tcaagcaggc cgctcaggca
      121 ggtgcatccc cgaggcctct tccaagccga tgctgtccac ctcgcgccgg gcctgcctca
      181 gaacaacacc tccctgatcg cgcagcctca ccgcggctct atagaacacg gtgtccctgg
      241 cattgtactt catgcagtta tctataatga gatcaaaatc ctcctcaaac tcatggaggt
      301 ttttataccc ttgagcttct aaccgtttcc tcattgtggc aaagtccatg ggatgtttaa
      361 tgtgatccaa ataatctggt acctccttca gactcacggg ctgcgcaaat atcctggcgg
      421 ggtccttgtc ttgcagctgg tccagcactg agcgcagcag caccgtcagc ggggtcagcc
      481 gcagctccat ggcgacctgc tccatggatt ggaagtacag gttctcggta cccagatcta
      541 caccagaaga atgatgatga tgatggtgca tatgtatatc tccttcttaa agttaaacaa
      601 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      661 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      721 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      781 cgccacttcg ggctcatgag cgcttgtttc ggcgtggt
//
