LOCUS       sequence-51866-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MMACHC
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctctgaatat tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgg agccgaaagt
      121 cgcagagctg aagcagaaga tcgaggacac gctatgtcct tttggcttcg aggtttaccc
      181 cttccaggtg gcatggtaca atgaactctt gcctccagcc ttccacctac cgctgccagg
      241 acctaccctg gccttcctgg tactcagcac gcctgccatg tttgaccggg ccctcaagcc
      301 cttcttgcag agctgccacc tccgaatgct gactgaccca gtggaccagt gtgtggccta
      361 ccatctgggc cgtgttagag agagcctccc agagctgcag atagaaatca ttgctgacta
      421 cgaggtacac cccaaccgac gccccaagat cctggcccag acagcagccc atgtagctgg
      481 ggctgcttac tactaccaac gacaagatgt ggaggctgac ccatggggga accagcgcat
      541 atcaggtgtg tgcatacacc cccgatttgg gggctggttt gccatccgag gggtagtgct
      601 gctgccaggg atagaggtgc cagatctgcc acccagaaaa cctcatgact gtgtacctac
      661 aagagctgac cgtatcgccc tactcgaagg cttcaatttc cactggcgtg attggactta
      721 ccgggatgct gtgacacccc aggagcgcta ctcagaagag cagaaggcct acttctccac
      781 tccacctgcc caacgattgg ccctattggg cttggctcag ccctcagaga
//
