LOCUS       sequence-51868-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ELAC1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca antttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtaatcatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgct ttcttgattg gaatgcttat caccataaaa
      181 tcttctgcta gagtcacttc ttggagatct aacactgatt cagcttgctt ttttagttct
      241 gcaatgccat ctgtttctcc ttctctggcc aaggcaactg gtttgtacct ctgactgaag
      301 tgagtcagaa ccagcctctt tgcacggcac aactttgcaa atgttgctgc catctgtggt
      361 gtgctgtggc catgctcctt tgctttgtcc atctgggcat catccagggt tgcttcgtgg
      421 atcaacaggt ctgcttcaaa gcacagtttt actcctccat cacccacaac cccagagcag
      481 tcacccaata tgcagatttt tcttccaaca ataggctttt ttaagacatc ttggggagaa
      541 attgtaaccc cattttccag aacaacagaa attccatttt tcagcttccc ataggcagga
      601 cctggtggaa caccaaggtc tttaagtttc tgtgcattga gtttacctgg gcgtttcttt
      661 tccacgactg aaaacccaaa tgagggaatt ctgtgaaaga ggcgaaatgc ttttacaaca
      721 aattgttcat catcaaacag aaggtatgag ttttcttctg agtctaacag gatagttctt
      781 ccttgttcct ctttgggagg actgtctgct ctattcacat gcgcaaattc ttttagttc
//
