LOCUS       sequence-51869-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLOD5
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(478..498)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(523..540)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             551..573
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    588..612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(612..631)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(613..631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(715..734)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc agtagttaga
      121 gacttcaatc aggttacggt ccgggtcacg aaaataaatg ctcataatcg gacctttagc
      181 acccgtacgc ggcaccggac cttcttcgat cgggacgtca catgctttca ggtgttgaat
      241 catttcttcc agcggcactt cggtgatcag gcaaatatcc agactgcccg ggaccgggtg
      301 tgctgctttc ggttcaaatt ctttaccaac ttcatgcagg ttgaatttct ggtcgccaaa
      361 acacagggct ttacgatctt ctttgaacgt cataacttcc atgcccagga ttttagagta
      421 gaacatcgtg gtgtctttga tgcttttaac ggtcatcacg atatgatcca ggcgcatgga
      481 ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat gatgatggtg
      541 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      601 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
      661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atct
//
