LOCUS       sequence-51871-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZFYVE16
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..25)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(484..504)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(511..681)
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             complement(691..708)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc aagcttgtcg acggagctcg
       61 aattcggatc cgtatccacc tttactgtca tagtccttca acacctggtt gtttaagtgc
      121 tgagactggc aaagttgctg gtgaaggtat actggatgtt tggttctcct gaggaggctg
      181 gacagtagta cattcatcag aatgattaga cttaagatta gaaccagttg gactcatcat
      241 cctttcaaat gcctgagctt tactaatagt ttcatagcag actacacata ctcttgcttc
      301 cttttctaga tattgcagtt tacacttcct attacaacag acaccacaaa atactttccc
      361 acatgctcgg caatggtgtc gccgtttggt aaaagtaaat ttgacttggc agttcataca
      421 gtttggagct tctgaatcag gaacccaagt aggctgtttc tggcccaaaa ccaagccttc
      481 catggattgg aagtacaggt tctcggtacc cttcggctgc gcatcgttca gcttcttcgc
      541 ttccgccagc aggttcgcgc tctggctcgg atcatcgcgc aggctgcgaa taaatgcgcg
      601 acgctgttcg cggttcaggt tcggcagatg gcgaatttca cggcgagctc ttcgacgctc
      661 cttgttgaac ttgttatcca caccagaaga atgatgatga tgatggtgca tatgtatatc
      721 tccttct
//
