LOCUS       sequence-51872-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ZFYVE16
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..232
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             239..259
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             718..735
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(788..806)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      802..849
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtggataac aagttcaaca aggagcgtcg aagagctcgc cgtgaaattc gccatctgcc
      121 gaacctgaac cgcgaacagc gtcgcgcatt tattcgcagc ctgcgcgatg atccgagcca
      181 gagcgcgaac ctgctggcgg aagcgaagaa gctgaacgat gcgcagccga agggtaccga
      241 gaacctgtac ttccaatcca tggaaggctt ggttttgggc cagaaacagc ctacttgggt
      301 tcctgattca gaagctccaa actgtatgaa ctgccaagtc aaatttactt ttaccaaacg
      361 gcgacaccat tgccgagcat gtgggaaagt attttgtggt gtctgttgta ataggaagtg
      421 taaactgcaa tatctagaaa aggaagcaag agtatgtgta gtctgctatg aaactattag
      481 taaagctcag gcatttgaaa ggatgatgag tccaactggt tctaatctta agtctaatca
      541 ttctgatgaa tgtactactg tccagcctcc tcaggagaac caaacatcca gtataccttc
      601 accagcaact ttgccagtct cagcacttaa acaaccaggt gttgaaggac tatgacagta
      661 aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac
      721 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      781 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      841 ggttttttgc tgaaaggagg aactatatcc ggattggcga a
//
