LOCUS       sequence-51873-e       12874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 12874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5b
FEATURES             Location/Qualifiers
     source          1..12874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..37
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     21..37
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        97..740
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     634..657
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     776..799
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     promoter        1962..2177
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2178..2981
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     3154..3170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3187..3418
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3814..4119)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3916..3935)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4464..5123)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5038..5057
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5124..5226)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(5371..5602)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7949..8195
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7964..7982)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(8235..8251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(8364..8952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8444..8463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9123..9914,9915..9983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9746..9765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9984..10088)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10205..11547
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11168..11215)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11581..11599
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11637..11659)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11759..11778
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11799..12080
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             12081..12755
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 atcaacgttg gtcattcaca caggaaacag ctatgaccat gtgacttgac tgatttcagt
       61 tcagtaggtc taaatcgtac gaatcatcgc agtttatcat tatcaatact cgccatttca
      121 aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag
      181 ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat
      241 ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag
      301 cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc
      361 ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac
      421 aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga
      481 tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc
      541 ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat
      601 tattccccta cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa
      661 atctatttct taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa
      721 acaccagaac ttagtttcga cggattctag aaaggatcct caacaaaaaa ttgttaatat
      781 acctctatac tttaacgtca aggagaaaaa actataatga ctaaatctca ttcagaagaa
      841 gtgattgtac ctgagttcaa ttctagcgca aaggaattac caagaccatt ggccgaaaag
      901 tgcggtattc caagtcttgc caagcccaga tccggagctt ggctgttgcc cgtctcactg
      961 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1021 gattcattaa tgcagctggc acgacaggtt tcccgactta atcgccttgc agcacatccc
     1081 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1141 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
     1201 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
     1261 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
     1321 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
     1381 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
     1441 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt
     1501 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg
     1561 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
     1621 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt
     1681 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt
     1741 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc
     1801 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta
     1861 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg
     1921 gtggttgaac tgcacaccga gctcaacccc cataccacct tttcaattca tcattttttt
     1981 tttattcttt tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac
     2041 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
     2101 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca
     2161 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
     2221 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
     2281 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
     2341 tttgtttact aaaaactcat gtggacatct tgactgattt ttctatggag ggcacagtta
     2401 agccgctaaa ggcattatcc gccaagtaca attttttact ctttgaagac agaaaatttg
     2461 ctgacattgg taatacagtc aaattgcaat actctgcggg tgtttacaga atagcagaat
     2521 gggcagacat tacgaatgca cacggtgtgg tgggtccagg tattgttagc ggtttgaagc
     2581 aggcggcaga agaagtaaca aaggaaccaa ggggcctttt gatgttagca gaattgtcat
     2641 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
     2701 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
     2761 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc
     2821 aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa
     2881 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
     2941 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
     3001 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattaccct
     3061 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     3121 gcgttaatgg gtttcatggt atcagagatc tttgtaaaac gacggccagt cttaagctcg
     3181 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa cacattgatg
     3241 agcaatgctt ttttataatg ccaactttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3301 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3361 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt
     3421 cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc
     3481 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
     3541 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
     3601 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
     3661 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
     3721 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
     3781 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
     3841 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3901 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3961 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     4021 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     4081 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
     4141 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     4201 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     4261 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     4321 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
     4381 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
     4441 agtaagccgg atccacgcgg cgtttacgcc ccgccctgcc actcatcgca gtactgttgt
     4501 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
     4561 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     4621 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     4681 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     4741 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     4801 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     4861 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     4921 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     4981 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     5041 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     5101 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     5161 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     5221 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
     5281 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
     5341 gcgtcgggtg atgctgccaa cttagtcgac tacaggtcac taataccatc taagtagttg
     5401 attcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     5461 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     5521 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     5581 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtaggac
     5641 tagtgctgga gggtgtacca ccaatctcga gtattcagca tgctggtaag ctagcttttc
     5701 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
     5761 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
     5821 ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
     5881 ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
     5941 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
     6001 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
     6061 acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
     6121 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
     6181 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
     6241 aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
     6301 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
     6361 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
     6421 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
     6481 cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
     6541 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
     6601 gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
     6661 cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
     6721 gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
     6781 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
     6841 ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
     6901 attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
     6961 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag
     7021 tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat
     7081 ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
     7141 cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg
     7201 ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
     7261 cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
     7321 gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct
     7381 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat
     7441 ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg
     7501 gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga
     7561 ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat
     7621 ctgccattgt cagacatgta taccccatac gtcttcccga gcgaaaacgg tctgcgctgc
     7681 gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
     7741 agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
     7801 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
     7861 agcccgtcag tatcggcgga atttcaactg agcgccggtc gctaccatta ccagttggtc
     7921 tggtgtcaaa aataatgata gtcatgagca tgtaattagt tatgtcacgc ttacattcac
     7981 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     8041 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     8101 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     8161 agaaggtttt gggacgctcg aaggctttaa tttgcgggcc ctgtcataca ttatcaacag
     8221 gacttcataa gcaaactggc cgtcgtttta catcggccgg taatacggtt atccacagaa
     8281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10081 gttccgcgca ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
    10141 accgacgacc gcgttcttcg gggcgaaaac tctccgcgca catttccccg aaaagtgcca
    10201 cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
    10261 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
    10321 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
    10381 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
    10441 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
    10501 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
    10561 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
    10621 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
    10681 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
    10741 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
    10801 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
    10861 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
    10921 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
    10981 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
    11041 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
    11101 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
    11161 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc
    11221 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
    11281 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
    11341 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta
    11401 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc
    11461 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa
    11521 tgctatcatt tcctttgata ttggatcata ctaagaaacc attattaatt aacctataaa
    11581 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    11641 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    11701 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
    11761 gcatcagagc agattgtact gagagtgcac catagatcaa cgacattact atatatataa
    11821 tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag ttatgacgcc
    11881 agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt acgtacaatc
    11941 ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag
    12001 gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac gtgattaagc
    12061 acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct ggtccattgg
    12121 tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat tccgatgctg
    12181 acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa
    12241 ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg
    12301 ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca
    12361 ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt
    12421 tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt
    12481 cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac
    12541 ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac
    12601 attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg
    12661 gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca
    12721 aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag tatttattta
    12781 agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga
    12841 gaaaataccg catcaggaaa ttgtaagcgt taat
//
