LOCUS       sequence-51881-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Diao F, White BH
  TITLE     A novel approach for directing transgene expression in Drosophila: 
            T2A-Gal4 in-frame fusion.
  JOURNAL   Genetics. 2012 Mar;190(3):1139-44. Epub 2011 Dec 29.
  PUBMED    22209908
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2012-03"; volume: "190(3)"; pages: "1139-44. Epub 2011 Dec 
            29"
COMMENT     Sequence Label: pC-attB-burs-mCD8-EGFP-T2A-Gal4
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..61
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             62..502
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 ggggcccgag ggccgcggca gcctgctgac ctgcggcgat gtggaggaga accccgggcc
       61 catgaagctg ctgagcagca tcgagcaggc ctgcgatatc tgccgcctga agaagctgaa
      121 gtgcagcaag gagaagccca agtgcgccaa gtgcctgaag aacaactggg agtgccgcta
      181 cagccccaag accaagcgca gccccctgac ccgcgcccac ctgaccgagg tggagagccg
      241 cctggagcgc ctggagcagc tgttcctgct gatcttcccc cgcgaggatc tggatatgat
      301 cctgaagatg gatagcctgc aggatatcaa ggccctgctg accggcctgt tcgtgcagga
      361 taacgtgaac aaggatgccg tgaccgatcg cctggccagc gtggagaccg atatgcccct
      421 gaccctgcgc cagcaccgca tcagcgccac cagcagcagc gaggagagca gcaacaaggg
      481 ccagcgccag ctgaccgtga gcatcgatag cgccgcccac cacgataaca gcaccatccc
      541 cctggatttc atgccccgcg atgccctgca cggcttcgat tggagcgagg aggatgatat
      601 gagcgatggc ctgcccttcc tgaagaccga tcccaacaac aacggcttct tcggcgatgg
      661 cagcctgctg tgcatcctgc gcagcatcgg cttcaagccc gagaactaca ccaacagcaa
      721 cgtgaaccgc ctgcccacca tgatcaccga tcgctacacc ctggccagcc gcagcaccac
      781 cagccgcctg ctgcagagct acctgaacaa cttccacccc tactgcccca tcgtgcacag
      841 cc
//
