LOCUS       sequence-51882-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HGS
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(779..799)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(824..841)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cncaatnttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcatccctcc gctttcctgt
      121 tcagctgctc gtagcagggc tcacacacgc gcacctcctt ctcgatgcca aacttgggga
      181 tggtggagta cttggaagaa cactttccac agaatatctg cccacacgcc cggcagtggt
      241 gcttacgggt catcaccccg aactgcaccc tgcagcggtg gcattcctca gcgtccaccc
      301 agtctggggc tctctcggca gcaaacatgg catcgctctc tttgaattct ggaaagacgt
      361 gcccctccac cttcatgatc tggtaggtgt cctggaccac cttgtacttg ggctcgttcc
      421 ggaaggcatg cgcccaggcc tggatcaggt acaggatctt gttacggacg tttacctcca
      481 cttgtctctt cagcaggtcc ttcagctcct ccatggtctg cttgttggcc acctcatcat
      541 gaactgtctg gccacagttc tttaccacag attccatgac ctccagggca tacaaggcga
      601 cgtgtgggtt cttgtcgttg actttcttct tgatggaatt cacagcatat tttgcttgtg
      661 tgtccccttg gcggatcagg tcgcagatct gcaaaatgga ctcccaatct gtctccaaca
      721 ggagctggct ggtcgccttg tctaggagac gctcgaaggt gccgctgcct cgccccatgg
      781 attggaagta caggttctcg gtacccagat ctacaccaga agaatgatga tgatgatggt
      841 gcatatgtat atctccct
//
