LOCUS       sequence-51883-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HGS
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             842..859
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt
       61 ctggtgtaga tctgggtacc gagaacctgt acttccaatc catggggcga ggcagcggca
      121 ccttcgagcg tctcctagac aaggcgacca gccagctcct gttggagaca gattgggagt
      181 ccattttgca gatctgcgac ctgatccgcc aaggggacac acaagcaaaa tatgctgtga
      241 attccatcaa gaagaaagtc aacgacaaga acccacacgt cgccttgtat gccctggagg
      301 tcatggaatc tgtggtaaag aactgtggcc agacagttca tgatgaggtg gccaacaagc
      361 agaccatgga ggagctgaag gacctgctga agagacaagt ggaggtaaac gtccgtaaca
      421 agatcctgta cctgatccag gcctgggcgc atgccttccg gaacgagccc aagtacaagg
      481 tggtccagga cacctaccag atcatgaagg tggaggggca cgtctttcca gaattcaaag
      541 agagcgatgc catgtttgct gccgagagag ccccagactg ggtggacgct gaggaatgcc
      601 accgctgcag ggtgcagttc ggggtgatga cccgtaagca ccactgccgg gcgtgtgggc
      661 agatattctg tggaaagtgt tcttccaagt actccaccat ccccaagttt ggcatcgaga
      721 aggaggtgcg cgtgtgtgag ccctgctacg agcagctgaa caggaaagcg gagggatgac
      781 agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga
      841 gcaccaccac caccaccact gagatc
//
