LOCUS       sequence-51888-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX3
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             334..354
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             355..372
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             373..396
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             446..463
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(516..534)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      530..577
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(701..720)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaagaagga gatatactat gaaagaagat ctcaaccttc gcttgaagaa attgactcat
       61 gctgccccct gcatgctgtt tatgaaagga actcctcaag aaccacgctg tggtttcagc
      121 aagcagatgg tggaaattct tcacaaacat aatattcagt ttagcagttt tgatatcttc
      181 tcagatgaag aggttcgaca gggactcaaa gcctattcca gttggcctac ctatcctcag
      241 ctctatgttt ctggagagct cataggagga cttgatataa ttaaggagct agaagcatct
      301 gaagaactag atacaatttg tcccaaagct gcagagaacc tctacttcca atcgcaccat
      361 catcaccacc atgattacaa ggatgacgac gataagtgag gatccgaatt cgagctccgt
      421 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      481 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      541 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      601 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      661 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      721 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      781 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtga
//
