LOCUS       sequence-51889-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX3
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(89..112)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(113..130)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(131..151)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    510..534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(534..553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(535..553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(637..656)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattcg gatcctcact tatcgtcgtc atccttgtaa tcatggtggt
      121 gatgatggtg cgattggaag tagaggttct ctgcagcttt gggacaaatt gtatctagtt
      181 cttcagatgc ttctagctcc ttaattatat caagtcctcc tatgagctct ccagaaacat
      241 agagctgagg ataggtaggc caactggaat aggctttgag tccctgtcga acctcttcat
      301 ctgagaagat atcaaaactg ctaaactgaa tattatgttt gtgaagaatt tccaccatct
      361 gcttgctgaa accacagcgt ggttcttgag gagttccttt cataaacagc atgcaggggg
      421 cagcatgagt caatttcttc aagcgaaggt tgagatcttc tttcatagta tatctccttc
      481 ttaaggttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      541 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
      601 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
      661 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
      721 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
      781 cggcggtgct caacggcctc aacctactac
//
