LOCUS       sequence-51893-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    622..646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(646..665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(647..665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(749..768)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtaatcatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgcc gggctcgcca tatcatcatc atccggcagc
      181 ggacccagtt ctttgcgttt ttctttcagc agtttttcca ggatatgcgc atcattatac
      241 acctggctac cttcttcgtt atagtgacgg gcgttacgaa acatcagttt catatcttca
      301 atcatacctt cttcgccggc atatttatcg ttacggatat tgtgttcaat aattttcaga
      361 tccatcggtt ccaggatgat tttataataa tccggataat cttttttgct cggtttcacc
      421 ataaacagat cacacaggcg gcgaccgctg cccggttcac gggcttccag caccacgtta
      481 aacagaattt tcatgcgctg tttacggata ttttttttgc ttttacgttt cgcgctgccg
      541 gtatcgctgg tcgcgctgct gatcatgcta tcacccatag tatatctcct tcttaaggtt
      601 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
      661 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      721 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      781 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggccc
//
