LOCUS       sequence-51896-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AMPH
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(825..845)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(870..887)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatccg tatccacctt tactgtcagg ccttgtcggc gtgctggtca
      121 cccagttttg tcatcacttc atacagtttg tggcaaagca ccgcaatttc cttatgaaac
      181 ttggcttcaa ggctggagac gtttttgaaa gtattaacat aaaatccaac tcgtcttgac
      241 cataatgatg gtaactcttc ttgtaagtca acgttaaact cttcaaacac tttctgtgct
      301 ttctgaaatt cttcttctgc cttagagatt cgactctcat ccttcctctt ggagctctgc
      361 agagcttcca gatggtggcg ggcactgtca tagtccacta gcttcctgct gcgcttggcg
      421 atgcgattct ttatgtcagg aaattgcccc aggtaggtat ccagtgttag caaggactca
      481 tccacgagtt tttgatggaa gtcttcccac agcacatcac atttctcacc aaccattttc
      541 acatcttccc gcccatacca gtcaggctca tagacttcat gcagcgactc tgtgagcttc
      601 atggaggcct cctgcatgcc tttgattgct gctaaatatc ctcggagttc tcgctgaagt
      661 ctggtaccct ctgcttcttg ccgtttgaag ttctggacat attcttcgaa ctgttcgtct
      721 tttgtctcat cagctttccc cagcttttgg aggacctttt cctgcgcgcg gttgagtcgc
      781 ttctggacgt tcttggcgaa gatgcccgtc ttgatgtcgg ccatggattg gaagtacagg
      841 ttctcggtac ccagatctac accagaagaa tgatgatgat gatggtgcat atgtatatct
      901 ccttcttaaa gttaaac
//
