LOCUS       sequence-51897-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AMPH
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             897..914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat
       61 tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc cgacatcaag
      121 acgggcatct tcgccaagaa cgtccagaag cgactcaacc gcgcgcagga aaaggtcctc
      181 caaaagctgg ggaaagctga tgagacaaaa gacgaacagt tcgaagaata tgtccagaac
      241 ttcaaacggc aagaagcaga gggtaccaga cttcagcgag aactccgagg atatttagca
      301 gcaatcaaag gcatgcagga ggcctccatg aagctcacag agtcgctgca tgaagtctat
      361 gagcctgact ggtatgggcg ggaagatgtg aaaatggttg gtgagaaatg tgatgtgctg
      421 tgggaagact tccatcaaaa actcgtggat gagtccttgc taacactgga tacctacctg
      481 gggcaatttc ctgacataaa gaatcgcatc gccaagcgca gcaggaagct agtggactat
      541 gacagtgccc gccaccatct ggaagctctg cagagctcca agaggaagga tgagagtcga
      601 atctctaagg cagaagaaga atttcagaaa gcacagaaag tgtttgaaga gtttaacgtt
      661 gacttacaag aagagttacc atcattatgg tcaagacgag ttggatttta tgttaatact
      721 ttcaaaaacg tctccagcct tgaagccaag tttcataagg aaattgcggt gctttgccac
      781 aaactgtatg aagtgatgac aaaactgggt gaccagcacg ccgacaaggc ctgacagtaa
      841 aggtggatac ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc
      901 accaccacca ccactgagat ccggctgcta acaaagcccg aaaaggaagc
//
