LOCUS       sequence-51899-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: VAT1L
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat ggagatgcgc gcggtggtgc
      121 tggctggctt cggggggctc aacaagctgc ggctcttcag gaaggccatg cccgagcctc
      181 aggacggcga gctcaagatc cgcgtcaaag cctgtggatt aaacttcatt gacttgatgg
      241 tgcgacaagg gaatattgac aaccctccca agactcccct ggtgccagga tttgagtgtt
      301 ctgggattgt tgaagctctg ggggacagcg tgaaaggata tgagattgga gaccgtgtca
      361 tggcatttgt caattacaat gcctgggcag aggtggtctg cacaccagtg gagtttgtct
      421 acaagatccc ggatgacatg agcttctcgg aggctgctgc attccccatg aacttcgtca
      481 cagcctatgt gatgctgttt gaagttgcca acctccggga agggatgtct gtgctcgtgc
      541 actcagctgg tgggggcgtg ggtcaagctg tggctcagct gtgttccact gtccccaacg
      601 tgactgtctt tggaacagcc tctactttca agcatgaagc aatcaaagac tctgtgaccc
      661 acctctttga cagaaatgca gactacgtgc aagaagttaa aagaatctct gctgaaggtg
      721 tggacatcgt tttggattgc ctctgtgggg acaacactgg aaaaggtctc agtcttctca
      781 aacccctggg aacctacatt ttatatggct catccaacat ggtaactgga gagaccaaga
      841 gcttcttcag ctttgcaaaa tcatggtggc aggtggagaa ggtgaacccc
//
