LOCUS       sequence-51900-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FABP9
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(491..511)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(536..553)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             564..586
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    601..625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(625..644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(626..644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(728..747)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatccgt atccaccttt actgtcacac cttttcgtag attctggtgc
      121 tgacaatatt attcatttta cattccacta ccattttttc atccacaatt tttcttttga
      181 ttgttgtctc tttgccaagc catttttgga cgtgaatcat tgagccattc tctaatgtta
      241 tggtgctctt tactttccgg ttgtctgctg tagtttcatc aaattcttcc cccagcttga
      301 aggagatctt agtgtcctgg aaagaacttt ctgttcttat ggtcatcatt ttcccatcaa
      361 cactaatagt tactgtcggt ttcactaacc ctgccatgtt ccgggctgcg aaattcactc
      421 ccagttcttt catgtaatcc tcaaagtttt cactggagac cagcttccag gttcccaaga
      481 agggctccat ggattggaag tacaggttct cggtacccag atctacacca gaagaatgat
      541 gatgatgatg gtgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
      601 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
      661 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      721 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      781 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actggttggg
      841 cgccatctcc
//
