LOCUS       sequence-51903-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CUL4B
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..232
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             239..259
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtggataac aagttcaaca aggagcgtcg aagagctcgc cgtgaaattc gccatctgcc
      121 gaacctgaac cgcgaacagc gtcgcgcatt tattcgcagc ctgcgcgatg atccgagcca
      181 gagcgcgaac ctgctggcgg aagcgaagaa gctgaacgat gcgcagccga agggtaccga
      241 gaacctgtac ttccaatcca tgccgaagct gcctgagaat tataccgatg aaacctggca
      301 gaaactgaag gaagccgtgg aagcaatcca gaacagtacc tcgattaaat acaatctgga
      361 agagctgtat caggccgtcg agaacctgtg ctcctacaaa atctctgcta atctgtataa
      421 gcagctgcgt cagatttgtg aggaccacat caaggcgcag attcatcagt tccgcgaaga
      481 cagcctggat tccgtgctgt ttctgaagaa aatcgatcgt tgctggcaga accactgtcg
      541 tcagatgatc atgattcgct caattttcct gtttctggac cgcacctacg tcctgcagaa
      601 cagcatgctg ccgagtatct gggatatggg tctggaactg ttccgtgccc atatcatttc
      661 tgaccagaaa gttcagaaca agaccatcga tggtattctg ctgctgatcg agcgtgaacg
      721 caatggcgag gctattgacc gttccctgct gcgctctctg ctgtcaatgc tgagtgacct
      781 gcagatttac caggattctt tcgaacagcg ttttctggaa gagaccaacc gcctgtatgc
      841 cgctgagggc cagaaactga tgcaggagcg cgaagttcct gaatacctgc accatgtgaa
      901 taagcgtctg gg
//
