LOCUS       sequence-51908-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CDKL1
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtaatcatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgct ggtttgtcgt gttcttttgc caaatcctct
      181 atttctctga tgttttcaaa atatgggtga tgcaacagct gttcacatgt cagcctttca
      241 gtagggtcca tgtggagaca gccctttagg agccccaggg caggataaga gatgtttggg
      301 aattttaatt caagtggttc catatcttca gggtctggaa ttttcactcc actgaagtac
      361 tgattcgtgc taaacacttg ctggtgccta ggaatgagat cccccaaggt cttcctaatc
      421 agatacagct gatccacatc cgattttcct ggccacagag gcactcctga cagcagctca
      481 gcaaagacac agccaattgc ccaaacatcc accggggggc cgtactgcgt gtcccccacc
      541 agcagctcag gggagcggta ccacctggta gccacttcgt cgtcatagta gtcactcggt
      601 ccagtcaaaa gccgagcaaa tccaaagtca caaagcttaa tcacggaatg tttcgtgatg
      661 aggatatttt ctggcttcac gtctctatgt atgcaattgt gtttatggca aaaatttaca
      721 gcttgcagtg tctgccaagt tatgctcttc acgagatgtt ctggtacccc tctttggtat
      781 ctgtccaact catggagaac tgtgtggtca caatattcaa acaccaggtg aagcctccgt
      841 ttcctcctga agacttccag gaggttaaca aggttgggat gcttgagttg cttgagcatt
      901 cgga
//
