LOCUS       sequence-51909-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BPTF
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(485..505)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(530..547)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             558..580
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    595..619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(619..638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(620..638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(722..741)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
       61 agcttgtcga cggagctcga attcggatcc gtatccacct ttactgtcaa tggctgcggc
      121 tggctttaaa gcctttcagt ttctgcacaa aaaagctttc cagaacttcc gcgcactgat
      181 aaaacgggct atcgctcgga ttataataac ggcagttatc aaaaattttg gtcatatccg
      241 ccacaaattc ggtcagtttt tcataatagc gacgctgaac acgttcttcc atggtcgcca
      301 gatccatcgg ttctttaatc acgccataat aatccggcgc atcattcgga tcaaccggtt
      361 ccagaaacgg ccacgccatt ttatgcgcct gcaggctgcg cagaacacgt ttcagacctt
      421 cataatcttt ttcggtcagc ggggtcagca cggtcatggc atcttcggtg ctctgacact
      481 gcatggattg gaagtacagg ttctcggtac ccagatctac accagaagaa tgatgatgat
      541 gatggtgcat atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt
      601 gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg
      661 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      721 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      781 gcttgtttcg gcgt
//
