LOCUS       sequence-51910-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BPTF
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             534..551
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(604..622)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      618..665
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(789..808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgtttacttt aagaaggaga tatacatatg caccatcatc atcatcattc ttctggtgta
       61 gatctgggta ccgagaacct gtacttccaa tccatgcagt gtcagagcac cgaagatgcc
      121 atgaccgtgc tgaccccgct gaccgaaaaa gattatgaag gtctgaaacg tgttctgcgc
      181 agcctgcagg cgcataaaat ggcgtggccg tttctggaac cggttgatcc gaatgatgcg
      241 ccggattatt atggcgtgat taaagaaccg atggatctgg cgaccatgga agaacgtgtt
      301 cagcgtcgct attatgaaaa actgaccgaa tttgtggcgg atatgaccaa aatttttgat
      361 aactgccgtt attataatcc gagcgatagc ccgttttatc agtgcgcgga agttctggaa
      421 agcttttttg tgcagaaact gaaaggcttt aaagccagcc gcagccattg acagtaaagg
      481 tggatacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc
      541 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      601 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      661 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      841 tcaagctc
//
