LOCUS       sequence-51911-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FKBP14
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(465..485)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(510..527)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             538..560
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    575..599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(599..618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(600..618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga
       61 gctcgaattc ggatccgtat ccacctttac tgtcaggatc ttggtccatt tcgaatctcc
      121 aggagatcaa tattaaatat cagtgtactt tctgggggaa ttttaccttt tccttctttt
      181 ccatagccca gagcaggagg aatgatgagc tttctcttct ctcctacaca cattcctttc
      241 aagccctggt cccaaccttt gagagcctcc aggatgccca gggtaaacca aatgggctga
      301 ccattgttat gtttgtgagt ggagtgaaat aaggagccgt ccttttctaa gtagccttca
      361 tagtggacca acatcaaatc ccctcctttg gtcttgcgat ggcagatgaa tggcttctgg
      421 agaacttcaa ttttcacttc tggttcaggg atcaaagccc ccatggattg gaagtacagg
      481 ttctcggtac ccagatctac accagaagaa tgatgatgat gatggtgcat atgtatatct
      541 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
      601 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat
      661 cgtggccggc atcaccggcg ccacaggtgc ggttgct
//
